Lab 10 Monitoring the chymotrypsin-catalyzed hydrolysis of p-nitrophenyl acetate

Objective: To track the chymotrypsin-catalyzed hydrolysis of p-nitrophenyl acetate using the spectrometers. Compound molecular FormulaMolecular WeightBoiling Point (°C) warming Point (°C)Density (g/cm3) p-nitrophenyl acetateC8H7NO4181.14--77-- acetonitrileC2H3N41.0582-450.786 Hydrochloric tartHCl36.46110-27.321.18 atomic number 11 phosphate dibasicNa2HPO4141.96--2500.5-1.2 Sodium phosphate monobasicNaH2PO4119.98------ chemic Structures p-nitrophenyl acetate acetonitrileHydrochloric AcidSodium phosphate dibasic Sodium orthophosphate monobasic   Observations: For this lab, I was partnered up with 5 other people, should have been 3 but there were two extra in the class. First, we picked astonished 2 cuvettes to make sure they were free of chymotripsin. We left the decolourise in the cuvettes for roughly 1 hour, rinsed them with DI water for another minute, and rinsed them with propanone so that they dry without too much trouble. The weight I was charge was 15mg, the other weights were 5mg, 10mg, and 20mg. Once one cuvette was dry, we proceeded to make a zero for the spectrometer. We added 2 portions of 940uL of the phosphate wing and 100uL of p-nitrophenyl acetate. We utilise this insight to zero our spectrometer.

Next, we each prepared our samples respectively and obtained the graph charge to our concentration of chymotrypsin. We used labtop 002, everything should be in the JABS folder. Data: clean Cuvettes term bleach left in cuvette~1 minute Time rinsed with DI water~1 minute Preparation of render Assigned weight15mg nitt y-gritty HCl in microcentrifuge tube1000uL ! total phosphate buffer in cuvette-940uL=1880uL total -940uL Amount p-nitrophenyl acetate in cuvette100uL Amount chymotrypsin solution used20uL Laptop 002JABS folder   Conclusions: 1.What are the typical pKas of aspartate, histidine, and serine? a.The pKa of aspartate is 3.8 COOH speciate is 3.1 amine group is...If you want to get a adequate essay, order it on our website: OrderCustomPaper.com

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